I-Compartmentation ingenye yezimpawu zamaseli e-eukaryotic, futhi izinqubo ezahlukene ze-biochemical zingahlukaniswa ngesakhiwo sendawo. Ukuhlaziywa kwe-Microscopy kanye ne-mass spectrometry kungasetshenziswa ukuhlaziya i-proteome yama-organelle ahlukene, kodwa kusenzima ukusebenzisa ngokuqondile lezi zindlela ezimbili ukukhomba i-interanomics ezingxenyeni eziningi ze-intracellular.
Ukuze kuhlonzwe ama-interromes ezindaweni ezihlukene kumaseli omuntu, ngo-June 2, 2021, iqembu lokucwaninga lika-Anne-Claude Gingras le-Lunenfeld{3}}Tanenbaum Institute e-Canada likhiphe iphepha elinesihloko esithi A proximity{4}}dependent biotinylation map of a humanticent map of a humanticent marked by a humanticent marked by a humanticent maps asungulwa.
Ii-biotin{0}}indlela yokulebula encike ekutheni isondele ingasetshenziswa ukuchaza indawo engaphakathi kweseli ehlala amaprotheni kuseli. Isibonelo, ubuchwepheshe be-BioID busebenzisa i-biotin ligase BirA* (* isho i-mutant), equkethe ukuguqulwa kwesayithi le-R118G, engasetshenziswa "njengesithiyo" ukuze ihlanganiswe nephrotheni eqondiwe futhi ivezwe kumaseli akhulisiwe noma ohlelweni lwamangqamuzana amaningi. in. Iphrotheni ye-biotinylated ingase ibanjwe yi-streptavidin, futhi iphrotheni esebenzisanayo ingahlaziywa ngokuhlonza i-mass spectrometry. Njengoba isilinganiso sobubanzi bephrotheni esiyiglobula singu-5-10nm, irediyasi yokulebula yalobu buchwepheshe ingalebula ngokuqondile i-biotin iphrotheni esebenzisanayo. Njengamanje, ubuchwepheshe be-BioID busetshenziswe ngempumelelo ukuchaza ukwakheka kwama-protein complexes amaningi ahlukene, ukuhlonzwa kokubopha ulwelwesi kanye nenhlangano yendawo yama-organelle angenalwelwesi.
Ukuze kuthuthukiswe ukuhlonza i-proteome esebenzisanayo kumaseli aphilayo womuntu, ababhali basebenzise ubuchwepheshe be-BioID ukulebula amaprotheni angama-234 e-intracellular ezingxenyeni zamaseli ahlukene angama-32 ngokuthi "ukudla" ukuze kubanjwe amaprotheni asebenzisanayo ukuze kusungulwe Isakhiwo senhlangano yamaprotheni kumaseli omuntu ophilayo. Iphrotheni ngayinye yokuyenga izolebula ngokuthi i-BirA*, bese kuzosungulwa umugqa wokubonisa ozinzile wamaseli e-HEK. Ngokuqhathanisa amaprotheni asebenzisanayo ahlonziwe nokulawula okungalungile, abalobi bahlola ukwethembeka kwezinto zokusebenzisana ezibanjwe. Isamba samakhandidethi amaprotheni angama-192 aphumelele ukuhlolwa kwekhwalithi, futhi kwahlonzwa isamba sokusebenzisana okuyizi-35,902. Isici sesenzo.
Uma ufuna isaphulelo esikhulu noma isampula yamahhala , sicela ukhululeke ukuxhumana :liu@wellgreenxa.com,Siyabonga.Sinesitoko esanele esingasithumela.
