Muva nje, ithimba le-algae ne-algae biotechnology e-Institute of Oceanology ye-Chinese Academy of Sciences lenze inqubekelaphambili ekuthuthukisweni kwezinsiza ze-astaxanthin ezivela ku-chlorella ebomvu. Kutholwe ukuthi izindlela eziningi -ezincike ezithombeni ze-metabolic, ezifana ne-anaerobic respiratory glycolysis (EMP), i-aerobic respiratory tricarboxylic acid cycle (TCA), pentose phosphate pathway (PPP) kanye ne-mitochondrial respiration alternate oxidase pathway (AOX), zinemiphumela ebalulekile yokulawula ku-astaxanthic synthesis. Imiphumela yashicilelwa emaphepheni amathathu ocwaningo ku-Bioresource Technology.
I-Astaxanthin ibomvu ngokugqamile, inombala onamandla kakhulu, amandla e-antioxidant kanye nemisebenzi ehlukahlukene yebhayoloji, futhi inethuba elibanzi lokufaka isicelo emkhakheni wokudla okunomsoco nokudla kwezempilo, imithi nezimonyo.I-Erythrochlorella iwuhlobo-lwelwelwe oluluhlaza oluneseli elilodwa elicebile i-astaxanthin, futhi liwumthombo ongcono kakhulu webhayoloji ukukhiqiza i-astaxanthin yemvelo emhlabeni. Phambilini bekukholelwa ukuthi i-astaxanthin biosynthesis yayiqhutshwa ikakhulukazi ukukhanya okuqinile futhi ibangelwa ukushoda kwezakhi. Iningi lezifundo zasekhaya nasemazweni angaphandle zigxile ezinqubweni ze-anabolic ezincike ngesithombe (njenge-photosynthesis, i-photoprotection kanye nokukhula kwamangqamuzana), futhi kwakunganakwa kancane ekuboleni nasekuphefumuleni okudlayo. Naseminyakeni yakamuva, i-photorespiration ne-chloroplast respiration kuphela okuhilelekile, kuyilapho indima{4}}yendlela ye-metabolic engancikile esithombeni ku-astaxanthin synthesis nokunqwabelana ku-S. novosa ibingavamile ukubikwa.
Ithimba lithole futhi laqinisekisa ukuthi izindlela eziningi{0}ezincike esithombeni zokuphefumula zingalawula ngempumelelo ukuhlanganiswa kwe-astaxanthin nokunqwabelana. Ucwaningo luthole ukuthi enye indlela ye-oxidase yomsebenzi wokuphefumula we-mitochondrial kanye nokubomvu kwatholakala ukuqoqwa kwe-astaxanthin kwaba ukuhlobana okungalungile, lapho ngemva kokuba indlela ehlongozwayo ivinjelwa, ihlobene eduze nokuphefumula phakathi kwe-metabolite pyruvic acid kanye ne-glyceraldehyde 3 phosphoric acid okuqukethwe kwathuthukiswa kakhulu, ngesikhathi esifanayo isizukulwane samandla se-NAstimulatePH. yezinhlobo ze-oxygen esebenzayo, Ngakho-ke, i-astaxanthin synthesis ingakhuthazwa. Kuphakanyiswa ukuthi ukulawulwa kokuphefumula kungakhuthaza i-astaxanthin biosynthesis kanye nokuqongelela.
Ngaphezu kwalokho, siphinde saqinisekisa ukuthi umjikelezo we-TCA, ihabhu elimaphakathi le-metabolism yamandla ekuphefumuleni okungancikile{0}esithombeni, ungahlinzeka ngamathambo e-carbon skeleton ye-astaxanthin synthesis futhi akhuthaze ukunqwabelana kwe-astaxanthin kumaseli ngokuyinhloko ngokungeza ama-metabolite amaphakathi okuphefumula. Indlela yayo yokulawula imi kanje: I-Metabolites ephakathi kwe-fumaric acid ingathuthukisa ukuphefumula kwe-EMP, i-TCA kanye ne-PPP izindlela ezintathu eziyinhloko, yenza i-pyruvic acid ne-glyceraldehyde 3 phosphoric acid amazinga anda kakhulu, ngakolunye uhlangothi, ngokukhuthaza i-astaxanthin synthesis precursor ye-isoamyl enestaxanthin synthesis futhi ikhuthaze ngokuqondile i-isoamyl enestapyrothesis yesandla, ukuhlanganiswa kwama-fatty acids, ngaleyo ndlela kusheshiswe inqubo ye-astaxanthin esterification, Ngokungaqondile ukukhuthaza ukuhlanganiswa kwe-astaxanthin. I-Oxaloacetate, enye i-metabolite yomjikelezo we-TCA, ingakhuthaza ngokuqondile ukuhlanganiswa kwe-astaxanthin ngokwandisa i-substrate namazinga e-NADPH. Uma kuqhathaniswa ne-fumaric acid, i-oxaloacetic acid inomphumela okhuthazayo osheshayo futhi obaluleke kakhulu, ikakhulukazi ngenxa yendlela yayo ye-transmembrane esebenza kahle kakhulu.
Imiphumela ithuthukisa uhlelo lokuthuthukiswa kwesikali se-astaxanthin esitholakala ku-astaxanthin esiyisisekelo se-basic theory and development technology, izosiza ukuthuthukisa i-bioreactor esekelwe ekulawuleni umjikelezo wamaseli kanye nobuchwepheshe obukhulu bokwakha ukukhanya obuvalekile obufana nemodi yokuthuthukiswa etholakele ebomvu, yenze izitshalo ze-photosynthetic zezitshalo zisebenze kahle, zikhuthaze izinga lokuthuthukiswa kwembonini lokukhuphuka. Ngesikhathi esifanayo, imiphumela yocwaningo ingasetshenziselwa ukubhekisela ocwaningweni nasekuthuthukisweni kwezinsiza zama-metabolites esibili njenge-carotene yamanye ama-microalgae.
