Amazinga Omnyango Wezolimo:
1 Phenol-I-Sulfuric Acid
Isimiso: I-Polysaccharides i-hydrolyze ibe yi-molecule ye-monosaccharide ngaphansi kwe-concentrated sulfuric acid, futhi iqede amanzi ngokushesha ukuze yakhe i-glucuronic aldehyde. Bese i-glucuronic aldehyde kanye ne-anthrone condensate ukwenza okuphuma kokunye kwe-glucuronic, okuluhlaza okwesibhakabhaka-okuluhlaza futhi ozinzile ngombala. Inokumuncwa okuyisici kokuthi ({3}}) nm. Okuqukethwe kwama-polysaccharides kunganqunywa ngemiphumela enembile kakhulu.
Ububanzi besicelo: Cishe wonke ama-carbohydrate anganqunywa, hhayi kuphela i-pentose ne-hexose, kodwa futhi wonke ama-oligosaccharides nama-polysaccharides, kuhlanganise isitashi, i-cellulose nokunye.
Izindinganiso ze-Pharmacopeia:
2 i-Sulfuric acid-isihlalo sobukhosi
Isimiso: Ngaphansi kwesenzo se-concentrated sulfuric acid, i-furfural noma i-hydroxymethylfurfural dehydrated kusuka kushukela ingafingqwa nge-phenol ukuze kwakheke isakhi esiwolintshi-ebomvu. Ukujula kombala wayo kuhambisana nokuqukethwe ushukela ebangeni lika-10-100 mg, futhi kukhona ukuphakama okuphezulu kokumuncwa ku-485 nm wavelength. Ngakho-ke, inganqunywa nge-colorimetry kule wavelength.
Ububanzi besicelo:
Indlela ye-Phenol ingasetshenziswa ekunqumeni ushukela we-methylated, amaPentosan nama-polysaccharides.
I-Wellgreen ingumnikezeli ochwepheshe wezitshalo ezikhishwe. Uma ungathanda ukwazi imininingwane eyengeziwe, wamukelekile ukuthi usithinte.
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