Izingxenye ezisebenzayo zemithi ye-herbal yaseShayina ziyisisekelo esibonakalayo somuthi waseShayina ukusebenzisa ukusebenza kahle kwawo, futhi iwumgogodla wokulawula ikhwalithi yawo. Ucwaningo lwe-Pharmacological eminyakeni yamuva lubonise ukuthi i-Gynostemma saponin iyingxenye yokwelapha eyinhloko ye-Gynostemma pentaphyllum, ukwakheka kwayo kwamakhemikhali okuyisisekelo i-dammarane{1}}uhlobo lwe-tetracyclic triterpenoid, futhi inemiphumela eminingi yemithi: 1. Ingakhuthaza ukulala nokukhathazeka; 2. Kungehlisa ushukela namafutha egazini; 3. Iphinde ibambe iqhaza elibalulekile ekwelapheni nasekuvimbeleni umdlavuza nezinye izifo ezingamahlalakhona. Ngakolunye uhlangothi, i-Gynostemma pentaphyllum extracts inothile ngama-flavonols afana ne- kaempferol ne-quercetin, nayo enemiphumela emihle ekuvimbeleni ukuvuvukala nokuvimbela ukukhula kwamangqamuzana omdlavuza. Lokhu kubonisa ukuthi i-Gynostemma Gynostemma saponins nama-flavonols ku-Gynostemma pentaphyllum ayizingxenye ezisebenzayo ze-Gynostemma pentaphyllum.
Lolu cwaningo lwenze uhlaziyo lwe-genome-olubanzi lwe-R2R3{{9}i-MYB yofuzo lomndeni we-Gynostemma pentaphyllum ukuze kuhlonzwe ama-MYB abalulekile ahilelekile ekulawuleni ama-Gynostemma saponins nama-flavonols emaqabunga e-Gynostemma. Isamba sezakhi zofuzo ze-R2R3-GpMYB ezingama-87 zikhonjwe ku-Gynostemma Gynostemma genome, engahlukaniswa ngamaqembu amancane angama-32. Izakhi zofuzo eziningi ezinemisebenzi efanayo eqenjini elincane elifanayo zinesakhiwo esifanayo se-exon-intron kanye nokwakheka kwe-motif. Lawa ma-R2R3-GpMYB asatshalaliswa ngokungalingani kuma-chromosome angu-11. Lolu cwaningo luthole ukuthi ukwanda komndeni wofuzo lwe-R2R3-GpMYB ku-genome ye-Gynostemma kwakubangelwa kakhulu ukuphindaphindeka kwezigaba. Ukuhlaziywa kwe-Ka/Ks kubonise ukuthi iningi lofuzo oluyimpinda lwe-R2R3-GpMYB selikhethiwe ukuhlanzwa.
Ukuhlonza ukuthi yiziphi izakhi zofuzo ze-R2R3-GpMYB ezingase zibandakanyeke ekulawuleni i-Gynostemma saponin kanye ne-flavonol biosynthesis emaqabunga e-Gynostemma, sithole ukuthi kunezinhlobo ezintathu zofuzo ze-R2R3-GpMYB (GpMYB60, GpMYB80 kanye ne-GpMYB81 ehlobene ne-biosynthesis ehlobene nenethiwekhi ye-biosynthesis) I-Gynostemma saponins nama-flavonols, futhi ingase ibambe iqhaza ekulawuleni i-biosynthesis ye-Gynostemma saponins nama-flavonols. I-qRT-Imiphumela ye-PCR ibonise ukuthi i-GpMYB81 yavezwa kanyekanye ne-Gynostemma saponin biosynthesis pathway genes GpFPS1, GpSS1, GpSE2 kanye ne-GpOSC1 kanye ne-flavonol biosynthesis pathway genes Gp4CL, GpCHS, GpCHI, GpFLSSH kanye ne-GpFLSH. Ukwengeza, ngokuhlolwa kweyeast ye-hybrid eyodwa kanye ne-dual luciferase, kwaqinisekiswa ukuthi i-GpMYB81 ingabophezela kubagqugquzeli be-Gynostemma saponin biosynthesis pathway gene GpFPS1 kanye ne-flavonol biosynthesis pathway gene GpFLS futhi isebenze ukuvezwa kwabo.
Umfanekiso 1 Ukuhlaziywa kokuqala kwendlela yokulawula ye-GpMYB81
Sengiphetha, imiphumela yalolu cwaningo ihlinzeka ngolwazi olusha lokuhlaziya indima yofuzo lwe-R2R3-GpMYB ekulawuleni izingxenye ezisebenzayo ze-Gynostemma pentaphyllum kanye nokuhlaziywa okwengeziwe kokusebenza komndeni wofuzo we-R2R3-GpMYB, futhi inikeze isisekelo esibalulekile sethiyori sokulawulwa kwe-biosynthesis yezingxenye ezisebenzayo ze-Gyntaphylum pentaphyllum.
