Muva nje, i-Horticulture Research ishicilele ku-inthanethi iphepha locwaningo elinesihloko esithi N-glucosyltransferase GbNGT1 kusuka ku-ginkgo igcwalisa i-auxin metabolic pathway ngumcwaningi u-Liu An ovela ku-Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences.
Ukuhlaziywa kwe-metabolic kuthole ukuthi izinhlobo ze-ginkgo ezingu-58 noma izinhlobo ezivela e-China nase-Japan zinothiswe kakhulu kumkhiqizo wetheminali ye-auxin IAA-ASP-N-iglucoside, enesisindo esomile esingafika ku-4.13 mg/g. Ngaphezu kwalokho, ngokuhlaziywa komsebenzi we-transcriptome kanye ne-enzyme, i-N-glycosyltransferase GbNGT1 engase ibangele i-IAA ne-IAA{8}}i-ASP ikhonjwe. I-Auxin iyisilawuli esibalulekile sokukhula kwezitshalo. Lesi sihloko sembula ukuguqulwa kwe-glycosylation{11}}kwendawo ye-auxin, eqeda umzila we-auxin metabolism.
Umbhali uhlole ama-indoles nama-flavonoid angu-52 futhi wathola ukuthi i-enzyme ishintsha ngokuqondile indawo ye-N ye-IAA-AA ne-IAA. I-enzyme inezici zokumelana ne-asidi, ukuzimela kwe-ion yensimbi kanye nokuzwela okuphezulu kwezinga lokushisa. I-molecular docking kanye nesayithi-i-mutagenesis eqondisiwe ithole ukuthi i-mutant E15A ilahlekelwe ngokuphelele ikhono layo le-IAA-i-Asp ne-IAA. Ababhali baphinde baqinisekisa imisebenzi yabo kugwayi. Ngesikhathi esifanayo yethula i-AtGH3.8 kanye ne-GbNGTs (noma ama-NGTs alungisiwe) ukuze uthole i-biosynthesis esuka ku-IAA ukuya ku-IAA{10}AA-N-glucoside kugwayi.
Kusukela i-IAA O-i-glycosylation enzyme yokuqala i-IAAGLU yashicilelwa kuSayensi ngo-1994, abacwaningi bathole imiphumela eminingi emkhakheni wokuthi i-IAA O-glycosylation ikuthinta kanjani ukukhula nokuthuthuka ngokulawula ibhalansi eguquguqukayo ye-IAA. Nokho, ucwaningo lwamanje olumayelana-nendlela ye-glycosylation kanye nokukhula nokuthuthukiswa kwe-IAA cishe akunalutho. Leli phepha lithole ukuthi i-IAA yezitshalo ze-GbNGT1 OE yayiphansi kakhulu kunohlobo lwasendle, kodwa isignali ye-IAA eyathinta ithiphu yezimpande yathuthukiswa kakhulu; ukuhlolwa kokukhula kwezimpande kutholwe ukuthi ubude bezimpande zesitshalo se-GbNGT1 esivezwa ngokweqile bushesha kakhulu kunohlobo lwasendle. Lolu cwaningo lwandisa ubudlelwano phakathi kwe-IAA N-yegatsha le-glycosylation nokukhula nokuthuthuka.
UDkt. Yin Qinggang, i-Institute of Chinese Materia Medica, i-China Academy of Chinese Medical Sciences, ungumbhali wokuqala wephepha, kanti umcwaningi uLiu An ungumbhali ohambisana naleli phepha. Abafundi abafundela ubudokotela u-Zhang Jing, izingcweti u-Wang Shuhui, kanye noDkt. Cheng Jintang babambe iqhaza kulolu cwaningo. UDkt. Sun Limin waseShandong Agricultural University kanye nomcwaningi uMa Lianbao wasePizhou Ginkgo Research Institute nabo benze igalelo elibalulekile kulesi sihloko. Lolu cwaningo lusekelwe yi-Beijing Municipal Natural Science Foundation kanye noHlelo Lukazwelonke Olubalulekile Locwaningo Nokuthuthukiswa.
