I-Eugenol (4-i-Allyl-2-i-methoxyphenol) iyisigaba esibalulekile se-benzene ring/phenylpropionic acid volatiles etholakala kabanzi ezimbalini zezitshalo nasezithelo ezivuthiwe, futhi inemiphumela ehlukahlukene yebhayoloji nemisebenzi yokunakekelwa kwezempilo. I-Eugenol ingadonsa impova yebhayoloji futhi isakaze imbewu, ivikele izilwane ezidla uhlaza, futhi ingasetshenziswa njenge-ejenti evikelayo ukuvimbela ukuhlasela kwezinambuzane ezinjengama-pathogen; I-Eugenol futhi inomsebenzi wokulwa ne-antibacterial, antibacterial, anti-insect kanye ne-flavour, futhi ithole ukunakwa okuseduze okuvela kubacwaningi{4}} Ngesikhathi esifanayo, i-eugenol iyingxenye ebalulekile yephunga lezithelo ze-strawberry, kodwa okuqukethwe kwayo kusithelo samasitrobheli esilinywe nge-octoploid kuphansi kakhulu kunaleso esisitrobheli sohlobo lwasendle lwe-diploid. Kuze kube manje, indlela ye-biosynthetic ye-eugenol iqondwa kakhulu ezitshalweni, futhi iningi labalawuli be-phenylpropanoid pathway abahlonziwe (njenge-R2R3-MYB transcription factor FaMYB10 kanye ne-FaEOBII, i-DNA-binding zinc finger transcription factor FaDOF2) iye yaqinisekiswa emidlalweni yama-strawberries, ngenkathi ikhula indima ekuthuthukisweni kwezithelo. izithelo zamasitrobheli esigabeni esikhulu sezithelo eziluhlaza, kodwa akucaci ukuthi ukuqoqwa kwe-eugenol kulawulwa kanjani ngokombhalo kulesi sigaba.
Kuma-strawberry, i-eugenol synthase-izakhi zofuzo ze-encoding FaEGS1 ne-FaEGS2 kanye ne-cinnamyl alcohol dehydrogenase{3}}i-encoding gene FaCAD1 zibalulekile ekukhiqizeni i-eugenol. I-FaEGS1 kanye ne-FaEGS2 zivezwe kakhulu esithelweni esiluhlaza sitrobheli nezithelo ezivuthiwe, ngokulandelana. Kulolu cwaningo, ukuze kuhlonzwe izici zokuloba ezihilelekile ekulawuleni i-FaEGS1, ukuhlolwa kweyeast single{10}okuxubile kwenziwe kusetshenziswa umgqugquzeli we-FaEGS1 njengesiyengo, kanye ne-R2R3{17}}I-MYB transcription factor FaMYB63 yaqhutshekwa kwafundwa emiphumeleni yokuhlola. I-FaMYB63 yenziwe yaba ngokwendawo ku-nucleus, futhi ukuhlaziya kwe-RT-qPCR kwabonisa ukuthi inkulumo ye-FaMYB63 yayiyithishu- nesiteji-iqondile, ihambisana nokuqukethwe kwe-eugenol phakathi nokuthuthukiswa kwesithelo sama-strawberry, futhi yayivinjwe i-abscisic acid (ABA) futhi yenziwa isebenze yi-auxin (IA). I-FaMYB63 overexpression kanye nokuthuliswa okuphakathi kwe-RNAi kubangele izinguquko eziphawulekayo kumazinga alotshiwe ezakhi zofuzo ze-eugenol synthesis FaEGS1, FaEGS2 kanye ne-FaCAD1, okuphinde kwathinta ukunqwabelana kwe-eugenol. I-EMSA, yeast monohybrid, umsebenzi we-GUS kanye nokuhlolwa komsebenzi we-luciferase okukabili kubonise ukuthi ukulotshwa kwe-FaEOBII ne-FaMYB10 nakho kwakulawulwa yi-FaMYB63, kodwa hhayi ngokuphambene. Ihlanganiswe ndawonye, le miphumela ihlinzeka ngemininingwane emisha kunethiwekhi yokulawula ye-strawberry eugenol synthesis pathway.
