Muva nje, ithimba lika-Tan Renxiang waseNyuvesi yaseNanjing lishicilele iphepha lokucwaninga ku-National Science Review (NSR). Bahlongoza, baklama futhi baqinisekisa umqondo "we-rheology yokusabela" ku-vivo, futhi basungula indlela esekelwe kulokhu yokuhumusha i-asidi yesisu. Izinyathelo ezithile nezinqumo zokuguqulwa kwe-I3C ibe ama-molecule amabili e-antitumor (DIM ne-LTr1) ngamagciwane amathumbu kanye nezindlela izilwane ezincelisayo ezihlanganisa ngazo i-I3C ne-DIM engapheli.
Phakathi kwalawa mamolekyuli amabili alwa{0}}nomdlavuza, i-DIM iyingxenye eyinhloko yochungechunge lwemikhiqizo yezempilo emakethe, futhi iyikhandidethi elisha lokulwa{1}}nomdlavuza elingena ezivivinyweni zomtholampilo. Ngokungalindelekile, i-LTr1 ibonise imiphumela eminingi yokulwa nomdlavuza kune-DIM emigqeni eminingi yamaseli wesimila kanye namamodeli egundane.
Ithimba liphakamise ukuthi amanye ama-molecule (ikakhulukazi ama-intermediate asebenzayo) ekugelezeni kwe-metabolic yezinto eziphilayo kufanele abe nokwenzeka "kwamaslayidi". Ngokusekelwe kulo mqondo, indlela ye-"reaction flux derailing" (RFD) yasungulwa, okungukuthi, kusetshenziswa ama-analogue e-substrate afakwe ngokuzenzakalelayo ukuze "kuhlehlise" indlela yokusabela yasekuqaleni kanye nokuthwebula okuphakathi , ngaleyo ndlela kuchazwa izindlela nezindlela zokushayela{1}}zezinguquko eziningi.
Besebenzisa le ndlela, abacwaningi baveze ukuthi i-I3C iqale iguqulelwe ku-3,3'{3}}diindolylmethane (DIM) bese iba ngu-2-(indol-3-ylmethyl) emgudwini wokugaya ukudla kusetshenziswa ama-analog e-I3C anelebuli. ) -3,3'-diindolylmethane (LTr1) indlela: Endleleni ye-acidic engenhla yesisu, i-I3C ingakwazi ukukhiqiza i-DIM ngokusebenzisa izindlela ze-deformaldehyde (ezinkulu) kanye ne-carbon dioxide (ezincane), ezihlanganiswa ne-3- Methylene indole (3MI) ibhekana nokusabela kwe-Michael add to form LTr1.
Emzileni wamathumbu, i-I3C ekhishwe emifino ye-cruciferous ingaguqulwa ibe yi-DIM ne-LTr1. Ethunjini elikhulu, i-formaldehyde ingahambisana ne-indole ukuze ikhiqize i-I3C ne-DIM engapheli.
Ithimba liphinde lathola ukuthi emathunjini amakhulu, i-endogenous indole (umkhiqizo wokuwohloka we-tryptophan) ingabhangqwana ne-formaldehyde (i-metabolite evamile ye-methanol, i-creatine, ne-folic acid) ukukhiqiza i-I3C engapheli kanye ne-DIM, kanye nama-bacteria amathumbu Amaqembu adlale indima ebalulekile kule nqubo.
Kafushane, lo msebenzi uthole ukuthi i-LTr1 ingaba nenani lomuthi; ihlongozwa futhi yaqinisekisa umqondo wepheshana le-rheological elisebenzayo ku-vivo; kusungulwe izindlela zokuhlola ezifanele, ngaleyo ndlela kuvezwe indlela yokwenziwa engapheli yama-molecule omndeni we-I3C kanye nesisu Inqubo yokuguqulwa iye yaholela ekuqondeni okusha kokubaluleka kwemifino ye-cruciferous (ecebile ku-I3C).
