Besebenzisa uhlobo lwe-A transgenic nematode CL2006 njengemodeli, abacwaningi eqenjini lika-Chang Chen bengeze i-LBE ekudleni kwe-nematode E. coli OP50 ekugxiliseni okuthile phakathi nenkathi yezibungu ze-L4 ukuze baphenye izindlela ezisebenzayo nezamangqamuzana ze-LBE ekukhiqizeni nasekukhishweni okunobuthi A. I-LBE itholakale inciphisa kakhulu ukuhlanganiswa kwe-A futhi ngaleyo ndlela inciphise i-phenotype yokukhubazeka kwemisipha ye-CL2006 nematodes. Ngakolunye uhlangothi, i-LBE inomsebenzi oqinile we-antioxidant futhi inganqanda ukukhiqizwa nokuhlanganisa kuka-A ngokwenza kusebenze umzila we-SKN-o-1 ukuze uvimbele ukukhiqizwa kwe-ROS (uhlobo lwe-oxygen esebenzayo). Ngakolunye uhlangothi, i-LBE ingenza kusebenze i-mitochondrial unfolded protein response (mtUPR) kuma-nematodes e-CL2006. Ukuphazamisa ukuvezwa kofuzo olubalulekile lwe-mtUPR kubuyisela emuva i-LBE{13}}ukwehla okulamuleyo kumaleveli A futhi kuthuthukisa i-nematode muscle paralysis phenotype. Nokho, ukuphazamisa ukuvezwa kofuzo olubalulekile kwezinye izindlela zokonakala{14}}ezihlobene, njengesistimu ye-proteasome, indlela ye-autophagic kanye ne-endoplasmic reticulum unfolded protein response (ERUPR), akuzange kuvimbele ukwehla kwe-LBE{16}}kumaleveli A. Ngaphezu kwalokho, ukuphazamiseka kokuvezwa kofuzo lwe-G protein-coupled receptor FSHR-1 kuvimbe ukwenziwa kusebenze kwe-mtUPR nge-LBE. Ngakho, i-LBE ivimbela i-Aggregation futhi igcina i-protein homeostasis ngokusebenzisa i-FSHR-1-mediated activation ye-mtUPR, ngaleyo ndlela ivimbele ukulimala okuqhubekayo kwe-mitochondrial.
Lolu cwaningo lukhomba okokuqala ngqa ukuthi i-LBE yenza umphumela wokuvikela kuma-nematode wemodeli ye-AD ngokusebenzisa i-UPR ye-mitochondrial, inikeze indlela entsha yokuveza ukusebenza okubalulekile kwe-LBP ekuvimbeleni nasekwelapheni i-AD. Ngokungafani nezifundo zangaphambilini ze-UPR ku-AD ezigxile kakhulu ku-endoplasmic reticulum UPR, lolu cwaningo lunikeza ubufakazi obusha bendima ye-cytoprotective ye-UPR ye-mitochondrial phakathi ne-AD. Ngaphezu kwalokho, izifo eziningi ezihlukene ze-neurodegenerative zingabelana ngembangela evamile kanye nendlela ye-pathological - ukugoqa nokuhlanganisa amaprotheni. Ngakho-ke, lolu cwaningo luphinde lunikeze ireferensi entsha yokuvimbela nokwelashwa kwezifo ze-neurodegenerative ngokuthuthukisa i-UPR ye-mitochondrial.
