Isifo iCoronavirus 2019 (COVID-19), esaziwa nangokuthi isifo se-coronavirus{10}}19, sibangelwa ukutheleleka kwe-novel coronavirus (Severe acute Respiratory syndrome coronavirus 2, SARS-CoV-2), nenyumoniya njengesifo somtholampilo esiyinhloko. Kuyisifo esithathelwanayo esibalulekile esinezimpawu ezisongela kakhulu impilo yabantu nokuvikeleka kwezempilo yomphakathi. Ukuqubuka kwe-COVID-19 kuholele ngqo ekumisweni komnotho womhlaba, okube nomthelela omkhulu emnothweni wezwe, ezombusazwe kanye nendlela yokuphila yabantu, futhi kulethe nezinselelo ezinkulu nemithwalo ekunakekelweni kwezempilo. Ngaphezu kwalokho, ukuvela okuqhubekayo kwama-genomes aguqukayo ngokushesha kanye nezinhlobo ezihlukahlukene ze-SARS-CoV-2 akukhulisi nje kuphela ubunzima bomuthi wokugoma nokuthuthukiswa kwezidakamizwa, kodwa futhi kwenza imithi eminingi evikelayo ekhona kanye namasosha omzimba angasebenzi kancane kancane angasebenzi. Izidakamizwa okwamanje ezisetshenziswa emtholampilo ku-COVID-19 zingahlukaniswa zibe yizidakamizwa ze-biological macromolecular kanye nezidakamizwa ezincane ze-molecule. Izidakamizwa ze-Biomacromolecular ngokuyinhloko zingamasosha omzimba anciphisa amandla, futhi ikakhulukazi ziqondise iphrotheni ye-S njengento yokwelapha. Kodwa-ke, ngokuvela okuqhubekayo kwezinhlobo eziguquguqukayo ze-SARS-CoV-2, futhi izindawo zayo eziyinhloko zokuguqulwa zigxile kuphrotheni ye-S, abantu kufanele baphinde bahlole ukusebenza kahle kwalezi zidakamizwa ze-antibody, ngakho-ke izidakamizwa ezincane ze-antiviral seziphenduke inketho yokwelapha i-COVID-19. isiqondiso esibalulekile.
NgoMashi 14, 2022, iSmall Science ishicilele ukushicilelwa okuku-inthanethi okunesihloko esithi Oridonin inhibits SARS-CoV{4}}2 yiqembu locwaningo lika-Sun Litao, i-Sun Yat-sen University School of Public Health (Shenzhen), iqembu locwaningo lika-Zhou Huihao, i-Sun Yat{8}Iqembu locwaningo lwe-Chinese Pharmacy of the Academy of the Academy of the Academy of Isayensi. Iphepha locwaningo eliqondise i-3C{11}}njenge-protease. Lolu cwaningo luqondise i-3C-njenge-protease (3CLpro) ye-SARS-i-CoV-2, ngokusekelwe kulabhulali ye-molecule encane yemithi ye-Chinese herbal, ngokusebenzisana, umsebenzi we-enzymatic, ukuhlaziywa kwe-mass spectrometry, ukuhlaziywa kwesakhiwo sokuxazulula okuphezulu kwe-complex, kanye ne-anti-virus. Ochungechungeni lokuhlola, kwatholakala ukuthi i-Oridonin, umuthi wendabuko wamaShayina, ingabophezela ngokuhlanganyela ku-cysteine ye-145 (Cys145) ye-3CLpro, engenza i-3CLpro ingasebenzi, ngaleyo ndlela ivimbele iphrotheni eyandulela igciwane ekubeni i-hydrolyzed futhi ivimbele inqubo yokuphindaphinda kwegciwane.
I-Rubescensine A ihlose inoveli ye-coronavirus 3CLpro
I-Rubescensine A, njengomuthi wendabuko waseShayina, inohlu olubanzi kakhulu lwezinhlelo zokusebenza zomtholampilo. Ine-antibacterial, anti{1}}imiphumela yokuvuvukala kanye ne-analgesic. Ngaphezu kwalokho, inomphumela ohlukile wokulwa-nomdlavuza, okuhlanganisa umdlavuza we-nasopharyngeal, umdlavuza womminzo, umdlavuza wamaphaphu, umdlavuza wesibindi, umdlavuza webele, i-leukemia, njll{4}} Kulolu cwaningo, kutholwe ukuthi i-oridonin A ithola ukungezwani kwe-electrophilic classical nge-Cys145 ye-3CLpro, kanye ne-sulf1actylphilic group ye-Cyshydrphilic recylic acid nge , -i-olefin engagcwalisiwe ku-oridonin A ukuze yakhe Umkhiqizo ongeziwe we-Oridonin-I-Cys145 ivimba ukubophezela kwe-3CLpro ku-substrate, ngaleyo ndlela kufezwe injongo yokuvimbela igciwane.
Ukusabela okungeziwe kwe-Electrophilic kwe-oridonin A nge-2019-nCoV Cys145
Lolu cwaningo luthole ukuthi i-oridonin A ingasebenzisa-imiphumela ye-SARS-CoV-2 ngokukhomba i-3CLpro. Kuyathakazelisa ukuthi ukulandelana kwe-3CLpro kwezinhlobo eziningi ezintsha ze-coronavirus ezintsha eziguquguqukayo (kuhlanganise nezinhlobo zamanje zobhubhane) kutholwe ukuthi kulondolozwe kakhulu ngokulandelana kwezakhi zofuzo, futhi okuwukuphela kwezindawo ezimbili zoguquko ezivele kuze kube manje bezingaboshiwe yi-oridonin A. locus, okuphakamisa ukuthi i-Rubescensine Ahinstrating kufanele ibe nomphumela oqinile we-Obinstratory njengamanje.
