Phakathi kwezinto ezihlukahlukene eziyingozi ekukhuleni kwezitshalo, ukhula lulimaza kakhulu futhi lubangela ukulahlekelwa kwesivuno esiningi kunokulahlekelwa okuhlangene okubangelwa izifo zezitshalo nezinambuzane emhlabeni wonke. Uma ukhula lungalawulwa, cishe u-33%-53% wesivuno uzobhujiswa. Ngokwesiko, imithi yokubulala ukhula ibisetshenziswa ukususa ukhula endaweni enkulu{7}}etshalwa ngamakhambi aseShayina. Kodwa-ke, nakuba ama-herbicides amakhemikhali athinta ukukhula nokukhula kokhula, nawo anemiphumela emibi kakhulu noma emincane emakhambi aseShayina ngokwawo, okuzogcina kuthinte isivuno, ikhwalithi kanye nempumelelo yomtholampilo yamakhambi aseShayina. Ukwengeza, ukusetshenziswa{10}}kwesikhathi eside kwenani elikhulu lama-herbicides kungabangela ukhula ukuthi luthuthuke ukumelana, okwenza ukulawula ukhula kamuva kube nzima kakhulu. I-Chemosensitiation ibhekisela emiphumeleni enenzuzo neyingozi yama-metabolites esibili ezitshalo, amagciwane, isikhunta kanye nolwelwe ekukhuleni nasekuthuthukisweni kwezilwane zezolimo nezemvelo ze-ecosystem, ezibizwa nge-chemosensitised substances. Ukusetshenziswa kwezinto ezizwelayo njengemithi yokubulala ukhula etholakala ezitshalweni sekuyisihloko esishisayo socwaningo, njengoba ayinayo imiphumela eyinsalela noma enobuthi.
I-Mugwort iqabunga elomisiwe le-Artemisia argyi Levl. et Vant. womndeni wakwa-Asteraceae, onomthelela wokufudumala ukuya esikhathini nokumisa ukopha, ukuhlakaza amakhaza nokukhulula ubuhlungu; isetshenziswa ngaphandle ukuze ikhiphe umswakama futhi ikhulule ukulunywa. Kusukela kudala emibhalweni yezinkondlo yaseShayina yasendulo, kwabhalwa ukuthi amaqabunga e-moxa asetshenziselwa ukubangela umlilo, futhi incwadi yokuqala yezokwelapha e-China, i-Yellow Emperor's Classic of Internal Medicine, yabhala ukusetshenziswa kwamaqabunga e-moxa ekwelapheni i-moxibustion. Isayensi yezokwelapha yesimanje ibonise ukuthi amaqabunga e-mugwort anemiphumela ehlukahlukene yemithi efana ne-antibacterial, antiviral, haemostatic, antitumour, hepatoprotective and choleretic, antioxidant, suppressant, analgesic and anti{4}}anti{4}}inflammatory, hypoglycaemic and immunomodulatory, with phenolic acids, flavonoids kanye ne-sesquiterpeology yayo eyinhloko. Kodwa-ke, ithimba laqaphela emkhakheni ukuthi i-mugwort inokuncintisana okuqinile kwezindawo zemvelo futhi iwumphakathi ovelele onokhula oluzungezile olumbalwa kanye nezinhlobonhlobo zezinto eziphilayo eziphansi, okusikisela ukuthi i-mugwort ingase ibe nomthelela wezinzwa we-biological and ecological oqinisiwe. Phakathi naleso sikhathi, ekuhlolweni kwangaphambilini kokutshalwa kwemvelo kwamakhambi aseShayina, i-mugwort powder yayiklanyelwe ukusetshenziswa njengomanyolo we-chrysanthemum, i-echinacea ne-atractylodes, futhi kwatholakala ngengozi ukuthi ukwelashwa kwempuphu ye-mugwort ephakathi nendawo kwakunomphumela omkhulu wokuvinjelwa kwezinhlobo zokhula, inombolo kanye ne-biomass emikhakheni ye-echinacedes ne-echinacedesmum kuqhathaniswa ne-echinacedes ye-echinacedes ne-echinacedesmum kuqhathaniswa ne-chrysanthemum. ukulawula. Imiphumela iphakamisa ukuthi amaqabunga e-mugwort angase abe nemithelela ebalulekile yokubulala ukhula futhi akwazi ukuthuthukiswa njengesitshalo{8}}sokubulala ukhula, ngakho imiphumela yawo yokubulala ukhula yahlolwa ngokuhlelekile futhi isisekelo esibalulekile nendlela yokusebenza yaphenywa.
Ngokokuqala ngqá, ithimba lathola ukuthi ama-aqueous extracts of mugwort amaqabunga anomsebenzi oqinile wokuvimbela ukhula lwe-monocotyledonous Setaria viridis ne-Echinochloa crusgalli, kanye nokhula oluyi-dicotyledonous Portulaca oleracea kanye ne-Amaranthus retroflexus. Kusetshenziswa umsebenzi wokubulala ukhula njengomhlahlandlela futhi kuhlanganiswe ne-LC-MS ukuze kwenziwe ukuhlukaniswa okuhlosiwe, inhlanganisela ye-phenolic acid isochlorogenic acid A itholwe iyinto eyinhloko esebenza nge-chemosensitively ku-mugwort futhi yatholwa enhlabathini ekhulayo ye-mugwort, okubonisa ukuthi i-mugwort ingavimbela ukukhula kokhula ngokukhululwa kwe-isochlorogenic acid. Ngesikhathi esifanayo, i-spectrum ebanzi yomsebenzi ovimbela ukhula we-isochlorogenic A yaqinisekiswa ukuvinjelwa kokukhula kokhula emindenini eyisithupha ehlukene, okubonisa ukuthi i-isochlorogenic A ingasetshenziswa njengesibulala-zinambuzane esiluhlaza futhi ingase inikeze ukudla okucatshangelwayo kanye nogqozi lwemithi emisha yokubulala ukhula njengenhlanganisela yamaphayona.
Umsebenzi. 1 we-Fig-ukuhlukaniswa okuqondisiwe kwezinto ezisebenzayo ezithinta i-chemosensitive ezikhishweni ezinamanzi zamaqabunga e-mugwort
Umfanekiso 2 Ukuhlolwa{1}}komsebenzi obanzi we-spectrum herbicidal we-isochlorogenic acid A
Ukuhlaziywa kokusebenza komzimba kubonise ukuthi ukwelashwa kwe-isochlorogenic acid A kubangele amaqabunga okhula ashwabene, aphuzi futhi afe. I-microscopy ekhanyayo kanye ne-electron microscopy yembula ukuthi i-isochlorogenic acid A yabangela ukuncipha kwengqamuzana leqabunga le-epidermal cell, ukwanda kwamaseli emithambo yemithambo yemithambo kanye nokuncipha kwe-chlorophyll yangaphakathi; ulwelwesi lwamaseli luhlakazekile futhi luhlukaniswe odongeni lweseli kanye nama-organelles ahlakazekile.
Ithimba locwaningo lasebenzisa i-RNA-i-seq ukuze licacise izinguquko kumazinga alotshiwe okhula ngemva kofuzo lwe-isochlorogenic acid A. 716 oluvezwe ngokwehlukana, lapho ezingu-439 zazo zaziphezulu{3}}zilawulwa futhi ezingu-277{5}}zilawulwa{5}}. "Ukuhlaziywa kokunothisa kwe-KEGG kwembula ukuthi ukuvinjwa kwendlela "ye-diterpenoid biosynthesis" kuyindlela ebalulekile yokwenza umsebenzi wokubulala ukhula we-isochlorogenic acid A, evimbela ikakhulukazi ama-weed gibberellins kanye ne-phytoplankton. Lokhu kuqinisekiswe yi-RT-qPCR nezilinganiso zokuqukethwe kwe-gibberellin.
