Muva nje, ithimba lika-Peng Cheng elivela ku-State Key Laboratory ye-Southwest Chinese Medicine Resources yase-Chengdu University of Traditional Chinese Medicine lisebenzise-i-GC ehlosiwe-i-MS ne-UHPLC/Q-I-Orbitrap MS ehlanganiswe nokuhlaziya okuhlukahlukene nokungaguquki ukuze kuhlaziywe amaphrofayili e-metabolic ezingxenyeni ezihlukene ze-chuquoxidative ekhishwe ku-menxioooooxidant ekhishwe ku-menxioooooxidant ekhishwe ku-menxioooooxidant ekhishwe ku-menxiool ehlukahlukene umsebenzi, umsebenzi we-vasodilatory kanye nomsebenzi we-anticoagulant wezinto ezikhishwe ezingxenyeni ezahlukene zahlolwa. Lolu cwaningo lunikeza ireferensi ebalulekile yocwaningo olusimeme nokuthuthukiswa kwezinsiza zemithi yendabuko yamaShayina ukuze kuhlolwe inani elingase lisetshenziswe lemithombo yemfucumfucu yemithi yendabuko yaseShayina i-Chuanxiong, ukuze kubonakale "ukusebenzisa kahle izinto kanye nokuguqula imfucuza ibe ingcebo".
Izingxenye zokwelapha zendabuko ze-chuanxiong (Ligusticum chuanxiong Hort.) ziyi-rhizome, kanti iziqu, amaqabunga kanye nezimpande ezinothayela ezinothe ngezinsiza zingezezingxenye "ezingezona-zemithi", ezilahlwa ngokwesiko. Ucwaningo lwangaphambilini lweqembu labacwaningi luthole ukuthi iziqu namaqabunga e-Chuanxiong aqukethe inani elikhulu lama-phthalide anezakhiwo zamanoveli eziphumuza kakhulu imithambo yegazi. Ngokusekelwe kusakhiwo-socwaningo lobudlelwano bomsebenzi, ithimba lisebenzise izivivinyo ze-electrophysiology ne-calcium imaging, kuhlanganiswe ne-homology modelling kanye nobuchwepheshe be-molecular docking, ukuze kukhethwe izimbangi ezintathu eziholayo ze-Cav1.2 kusukela kulabhulali ehlanganisiwe. Isikhala esikhulu socwaningo sokuthuthukiswa kwemithombo emisha yezidakamizwa (Bioorg. Chem., 2021, 107, 104523, chofoza ukuze ufunde ukuze uthole imininingwane). Kodwa-ke, ukusatshalaliswa kwezakhi ze-bioactive e-Chuanxiong akukacaci, futhi ukuhlukahluka kwezingxenye ze-phthalide kufanele kuqhutshekwe kuhlolwe. Kulolu cwaningo, izinhlanganisela ezingu-75 kuwoyela oguquguqukayo wezingxenye ezihlukene (amaqabunga esiqu, izimpande ezine-fibrous, ama-rhizomes) e-Chuanxiong aye abonakala nge-GC-MS. Kusetshenziswa ubuchwepheshe be-UHPLC/Q-I-Orbitrap MS ubuchwepheshe, isamba sezinhlanganisela ezingama-243, okuhlanganisa nezinhlanganisela ze-phthalide ezingu-95, zikhonjwe ekukhishweni kwe-methanol ezingxenyeni ezihlukahlukene. Ngenxa ye-isomerism esobala yezinhlanganisela ze-phthalide, imiphumela yekhwalithi esekelwe ekufanisweni kwesizindalwazi esivamile ayilungile. Ithimba labacwaningi lihlonze izingxenye ze-phthalide ngokusekelwe{22}}kusizindalwazi esakhelwe yona ilabhorethri, ucwaningo lwemibhalo kanye nokuhlaziywa kwezindlela eziqhekekayo, okuhlanganisa nokuhlanganisa okusha okungaba ngu-14. Ngokuvamile, ukwakheka kwamakhemikhali ezingxenyeni ezahlukene ze-Chuanxiong kuyafana, futhi i-phthalide iyingxenye eyinhloko yengxenye ngayinye. Kubalulekile ukuqaphela ukuthi ekukhishweni kwe-methanol, okuqukethwe kwe-monophthalide kwakuphakeme kuma-rhizomes kanye nezimpande ezine-fibrous, kuyilapho ama-phthalide dimers noma ama-phthalide trimers asakazwa kakhulu ngeziqu namaqabunga (Fig{26}}). Imiphumela yokuhlaziywa kwe-PCA ne-HCA ihlukanise amasayithi ahlukene ngamaqembu ahlukene (I-Fig. 4). Ama-metabolite ahlukile ahlolwe inani le-VIP, inani le-FC, nenani le-p futhi abonwa ngeso lengqondo kusetshenziswa imephu yokushisa (Umfanekiso 5). Imiphumela yabonisa ukuthi ukwakheka kwamakhemikhali ezingxenyeni ezahlukene zeChuanxiong kwakuhlukile. Ekuhlolweni komsebenzi wezingxenye ezihlukene, iziqu namaqabunga anomsebenzi ongcono kakhulu wokulwa ne-anticoagulant, izimpande ezine-fibrous zinomsebenzi oqinile we-vasodilatory, futhi ama-rhizome anomsebenzi obaluleke kakhulu we-anticoagulant (Fig. 6). Lona umphumela womehluko kuma-metabolites ezingxenyeni ezihlukene futhi kuveza Imvelo "yomzimba{35}}owodwa,{36}}obuningi{36}}obuningi bomuthi wendabuko wamaShayina. Ngokuvamile,{38}izingxenye ezingezona zokwelapha ze-Chuanxiong zinenani elikhulu lezinsiza zebhayoloji, futhi ukuhlukahluka kwesakhiwo kwe-phthalide kucebile, okubonisa amandla amakhulu ocwaningweni lwezinye izinsiza kanye nokuthuthukiswa kwemithombo emisha yemithi.
Ukusatshalaliswa kwezakhi zamakhemikhali ezingxenyeni ezihlukene ze-Chuanxiong. Amafutha Aguquguqukayo (a); I-Methanol Extract (b)
Izikolo ze-PCA kanye nemidwebo ye-HCA yamafutha aguquguqukayo kanye nokukhishwa kwe-methanol kwezingxenye ezahlukene ze-Chuanxiong. Imephu ye-PCA yamaphuzu kawoyela aguquguqukayo (a); Imephu yemiphumela ye-PCA ye-methanol extract (b); Imephu ye-HCA yamafutha aguquguqukayo (c); Imephu ye-HCA ye-methanol extract (d)
Imephu yokushisa yama-metabolites ahlukene emafutheni aguquguqukayo (a) kanye ne-methanol ekhishwe (b) yezingxenye ezahlukene ze-Chuanxiong
I-matrix yokuhlobana kwe-Pearson phakathi kwamanani e-IC50 wamazinga okulahla ama-radical mahhala e-DPPH ne-ABTS nokuqukethwe kwezingxenye ezihlukahlukene (a); imisebenzi ye-vasodilatory yezingxenye ezahlukene ze-Chuanxiong (b); imiphumela yezingxenye ezahlukene ze-Chuanxiong ku-APTT, PT kanye ne-TT coagulation isikhathi kumagundane (ce)
Kulolu cwaningo, i-Chuanxiong, into yokwelapha yangempela evela eSichuan, isetshenziswe njengesibonelo, futhi izindlela ezihlukahlukene zobuchwepheshe zasetshenziswa ukuze kuhlaziywe futhi kuqhathaniswe umehluko ezingxenyeni zamakhemikhali kanye nemisebenzi yezinto eziphilayo zezingxenye ezihlukahlukene. Treasure", enikeza ireferensi ebalulekile yocwaningo olusimeme nokuthuthukiswa kwezinsiza zemithi yendabuko yamaShayina. Ukutholwa kwevelu yemithombo yemfucuza yemithi yamaShayina kuzothuthukisa ukuguqulwa kwemboni yezinsiza zemithi yendabuko yaseShayina yezwe lami ibe yinsiza{2}}eyokonga kanye nemvelo{3}}imodeli yemboni yezomnotho eyindilinga enobungani.
